ABSTRACT
<p><b>OBJECTIVE</b>To investigate the effects and mechanisms of Gong-tone music on the immunological function in rats with the Chinese medicine syndrome of Liver (Gan)-qi stagnation and Spleen (Pi)-qi deficiency (LSSD).</p><p><b>METHODS</b>Twenty five male Wistar rats of SPF grade were randomly divided into 5 groups: normal group, model group, Xiaoyao Powder () group, Gong-tone group and combined group (the combination of Gong-tone and Xiaoyao Powder), with 5 rats in each group. The rat model for the Chinese medicine syndrome of LSSD was induced by chronic bandage and irregular diet. The course of treatment was 21 days. After the treatment, the levels of serum gastrin and IgG were detected by enzyme-linked immunoabsorbent assay (ELISA). Phagocytosis of macrophages was detected by the neutral red uptake assay and T cell proliferation was investigated by 3-(4,5-dimethylthiazolyl)-2,5-diphenyltetrazolium bromide (MTT) assay.</p><p><b>RESULTS</b>The serum gastrin, macrophage phagocytosis, IgG level and proliferation ability of T cells in the model group were significantly decreased compared with those in the normal group (P <0.05). Compared with those in the model group, the serum levels of gastrin, macrophage phagocytosis, IgG level and proliferation ability of T cells in Gong-tone, Xiaoyao Powder, and combined groups were significantly increased (P <0.05). The combined group was superior to either Gong-tone group or Xiaoyao Powder group.</p><p><b>CONCLUSION</b>Gong-tone music may upregulate the immunological function and play a role in adjuvant therapy in the Chinese syndrome of LSSD.</p>
Subject(s)
Animals , Male , Rats , Auditory Perception , Behavior, Animal , Body Weight , Cell Proliferation , Depression , Blood , Allergy and Immunology , Gastrins , Blood , Immunoglobulin G , Blood , Liver , Allergy and Immunology , Macrophages , Cell Biology , Music , Phagocytosis , Qi , Rats, Wistar , Spleen , Allergy and Immunology , Syndrome , T-Lymphocytes , Cell Biology , MetabolismABSTRACT
<p><b>OBJECTIVE</b>To investigate the effect of Nd:YAG laser on microtensile bond strength of single bond adhesive system for non-carious sclerotic dentin.</p><p><b>METHODS</b>Ten human molars with occlusal wearing were cut into equal halves (nearly 12 mm(2)), and randomly divided into experimental group and control group. The teeth in experimental group were processed with Nd:YAG laser (1 W, 10 Hz), and then applied with Scotchbond and filled with Z350 resin. In control group, the teeth were processed with single bond and filled with Z350 resin. The specimens were sectioned, and the microtensile bond strengths of each sample was tested by a universal testing machine.</p><p><b>RESULTS</b>The bond strength of the experimental group [(26.11 ± 1.62) MPa] was significantly higher than that of the control group [(22.27 ± 2.16) MPa], P < 0.05. Stero-microscope examination indicated that most of the fractures occurred in dentin-resin interface.</p><p><b>CONCLUSIONS</b>Nd:YAG laser can increase the microtensile bond strength of single bond adhesive system in non-carious sclerotic dentin.</p>
Subject(s)
Humans , Bisphenol A-Glycidyl Methacrylate , Dental Bonding , Dental Cements , Dental Stress Analysis , Dentin , Dentin, Secondary , Dentin-Bonding Agents , Lasers, Solid-State , Molar , Resin Cements , Tensile StrengthABSTRACT
<p><b>OBJECTIVE</b>To quantify the interleukin (IL)-1beta mRNA and IL-6 mRNA expression induced by lipopolysaccharides ([PS) extracted from Porphyromonoas endodontalis (P. endodontalis) in osteoblasts, and to relate P. endodontalis LPS to the bone resorptive pathogenesis in the lesions of chronic apical periodontitis.</p><p><b>METHODS</b>MG63 cells was pretreated with PD98059 or SB203580 for 1 h and then treated with P. endodontolis LPS for 6 h. The expression of IL-1beta mRNA and IL-6 mRNA were detected by reverse transcription polymerase chain reaction (RT-PCR) technique.</p><p><b>RESULTS</b>The production of IL-1beta mRNA induced by P. endodontalis LPS decreased in osteoblasts pretreated with PD98059. Both of the production of IL-1beta mRNA and JL-6 mRNA induced by P. endodontalis LPS decreased in osteoblasts pretreated with SB203580.</p><p><b>CONCLUSION</b>The synthesis of IL-1beta mRNA stimulated by Pendodontalis LPS in MG63 probably occur via extracellular signal-regulated kinase (ERK) 1/2 and p38 mitogen activated protein kinase (MAPK) signal transduction system. The synthesis of IL-6 mRNA stimulated by P.endodontalis LPS in MG63 probahly occur via p38MAPK signal transduction system.</p>